Direct Observation of Reduction of Met- and Ferrylmyoglobins in the Hemoglobin-Free Perfused Rat Heart 1

Abstract
It was demonstrated spectrophotometrically that in the hemoglobin-free perfused rat heart met-and ferrylmyoglobins formed upon infusion of sodium nitrite, and ethyl hydroperoxide were reduced to form oxymyoglobin with a half time of about 3 and 4 min, respectively. The rate of metmyoglobin reduction was about 30 nmol/min/g wet weight in the rat heart.