Expression of CMS-unique and flanking mitochondrial DNA sequences in Phaseolus vulgaris L.
- 1 March 1994
- journal article
- Published by Springer Nature in Current Genetics
- Vol. 25 (3) , 245-251
- https://doi.org/10.1007/bf00357169
Abstract
The expression of mitochondrial DNA sequences unique to a cytoplasmically male-sterile (CMS) line of Phaseolus vulgaris was investigated. RNA-blot hybridizations with strand-specific probes demonstrated CMS-unique transcripts (7.0, 6.8, 4.7, 3.3 and 2.8 kb) to be in the sense orientation with respect to the longest open reading frames within the CMS-unique region. Hybridizations revealed co-transcription of CMS-unique and upstream, atpA-coding sequences to generate the 6.8-kb RNA. However, hybridizations with CMS-unique and flanking DNA probes accounted for only 4.9 kb of the longest and most abundant (7.0 kb) CMS-unique transcript, providing indirect evidence for the involvement of a splicing process in the generation of this transcript. Sedimentation experiments demonstrated the association of 7.0- and 6.8-kb CMS-unique transcripts with polyribosomes in seedlings and floral buds of a CMS line and a line restored to fertility by the nuclear gene Fr2. However, steady-state levels of the 7.0- and 6.8-kb transcripts were decreased in the restored line relative to the CMS line.Keywords
This publication has 29 references indexed in Scilit:
- Nuclear Mutants of Maize with Defects in Chloroplast Polysome Assembly Have Altered Chloroplast RNA Metabolism.Plant Cell, 1993
- Regenerating good sense: RNA editing and trans splicing in plant mitochondriaTrends in Genetics, 1992
- PLANT MITOCHONDRIAL MUTATIONS AND MALE STERILITYAnnual Review of Genetics, 1991
- Multiple trans-splicing events are required to produce a mature nad1 transcript in a plant mitochondrion.Genes & Development, 1991
- The wheat mitochondrial gene for subunit I of the NADH dehydrogenase complex: A trans-splicing model for this gene-in-piecesCell, 1991
- Trans splicing in oenothera mitochondria: nad1 mRNAs are edited in exon and trans-splicing group II intron sequencesCell, 1991
- A 3' stem/loop structure of the Chlamydomonas chloroplast atpB gene regulates mRNA accumulation in vivo.Plant Cell, 1991
- The Texas Cytoplasm of Maize: Cytoplasmic Male Sterility and Disease SusceptibilityScience, 1990
- Fertility Restoration Is Associated with Loss of a Portion of the Mitochondrial Genome in Cytoplasmic Male-Sterile Common Bean.Plant Cell, 1990
- Formation of lariats and circles in self-splicing of the precursor to the large ribosomal RNA of yeast mitochondriaCell, 1986