Oligonucleotide Arrays from Aldehyde-Bearing Glass with Coated Background
- 26 April 2002
- journal article
- research article
- Published by Georg Thieme Verlag KG in Synthesis
- Vol. 2002 (06) , 816-824
- https://doi.org/10.1055/s-2002-25758
Abstract
Presented here is a method for preparing small DNA arrays on aldehyde-bearing glass slides. Immobilization involves reductive amination and employs oligonucleotides with 3′-terminal lysine residues, obtained in high yield from solid phase syntheses. Spot patterns are produced by protecting selected areas of the aldehyde slides with wax, coating the free surface with a methyl triethylene glycol derivative, and removing the wax with dichloromethane. The DNA arrays give better signal to noise ratios in hybridization experiments than slides without passified background.Keywords
This publication has 0 references indexed in Scilit: