Human Serumα2HS-Glycoprotein Modulatesin VitroBone Resorption*

Publisher Website
Google Scholar
Abstract
We previously isolated a family of bone-resorbing proteins from human cancer ascites fluid and established that the three purified bone-resorbing proteins were chemically and immunochemically related to each other and to α-2HS glycoprotein (α2HS). After this finding we purified the normal human serum counterpart of these ascites proteins and studied its effects on bone resorption. The bone-resorbing properties of normal human serum α2HS were examined in vitro over a wide dose range. This normal human serum glycoprotein had a biphasic effect on 45Ca2+ release from bone. More specifically, this protein stimulated bone resorption at the lower concentrations tested, with a maximum effect [treated over control ratio of 2.5 ± 0.30 (±se); P < 0.01] at 40 μg/mL. In contrast, at doses above 40 μg/raL, a sharp decline in calcium mobilization occurred, with a return to baseline occuring above 80 μg/mL. These results suggest that serum α2HS may participate in the regulation of bone metabolism in vivo.

This publication has 13 references indexed in Scilit: