Analytical isoelectric focusing of R factor-determined beta-lactamases: correlation with plasmid compatibility
- 1 February 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 125 (2) , 713-718
- https://doi.org/10.1128/jb.125.2.713-718.1976
Abstract
R factor-determined .beta.-lactamases [Escherichia coli] were investigated by analytical isoelectric focusing. The enzymes such as those specified by the R6K and RP4 plasmids (TEM-type enzymes) are notably homogenous in biochemical tests but 2 subclasses can be distinguished by isoelectric focusing. Three subclasses can be distinguished among the oxacillin-hydrolyzing enzymes, in good agreement with the classification based upon biochemical characteristics. The TEM-type .beta.-lactamases are promiscuously distributed among plasmids of a wide variety of compatibility groups, whereas the various oxacillin-hydrolyzing enzymes show some degree of correlation with compatibility.This publication has 17 references indexed in Scilit:
- R factors from ProvidenceMicrobiology, 2000
- The Use of Analytical Isoelectric Focusing for Detection and Identification of -LactamasesJournal of General Microbiology, 1975
- Origin of the TEM-beta-lactamase gene found on plasmidsJournal of Bacteriology, 1975
- Mobilization of plasmid-borne drug resistance determinants for transfer from Pseudomonas aeruginosa to Escherichia coliMolecular Genetics and Genomics, 1975
- R-factor-mediated beta-lactamases that hydrolyze oxacillin: evidence for two distinct groups.1974
- R-Factor-Mediated β-Lactamases That Hydrolyze Oxacillin: Evidence for Two Distinct GroupsJournal of Bacteriology, 1974
- Transposition of ampicillin resistance from RP4 to other repliconsMolecular Genetics and Genomics, 1974
- R Factors from Proteus rettgeriJournal of General Microbiology, 1972
- Resistance ofEscherichia colito Penicillins V. Physiological Comparison of Two Isogenic Strains, One with Chromosomally and One with Episomally Mediated Ampicillin ResistanceJournal of Bacteriology, 1968
- Penicillinase Synthesis Controlled By Infectious R Factors In EnterobacteriaceaeNature, 1965