Electrochemical detector for high performance liquid chromatography. IV. Analysis of fatty acids, bile acids and prostaglandins by derivatization to an electrochemically active form.

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Abstract
A high performance liquid chromatography (HPLC) procedure with an electrochemical detector (ECD) was developed for the analysis of fatty acids, bile acids and prostaglandins [PG]. These carboxylic acids were converted to p-hydroxyanilides by reaction with p-aminophenol in the presence of 2-bromo-1-methylpyridinium iodide and triethylamine. The anilides were oxidized in a 2-electron process and analyzed by reversed-phase HPLC-ECD. The detection limits were 0.5, 2 and 2 ng for stearic acid, chenodeoxycholic acid and prostaglandin[PG]F2.alpha., respectively. The method was applied to the determination of free fatty acids in guinea pig plasma and bile acids in human bile.