Mechanism and optimized conditions for PEG mediated DNA transfection into plant protoplasts

1 January 1989

journal article
research article
Published by Springer Nature in Plant Cell Reports

Vol. 8 (3) , 148-151
https://doi.org/10.1007/bf00716828

Abstract

Experimental conditions influencing DNA uptake efficiency by maize protoplasts in polyethyleneglycol (PEG) mediated transfection experiments have been studied systematically. The data provide evidence that the extracellular DNA is precipitated efficiently by combined action of PEG together with divalent cations and DNA is taken up by the plant protoplasts in the precipitated form. The particle size is strongly effected by the pH of the PEG solution. At optimal pH 6– 6.5 a very fine and homogenous precipitate forms in presence of Ca²⁺ and Mg²⁺ ions and is efficiently incorporated by maize and rice protoplasts.

Keywords

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