Hepatocellular Carcinoma After Thorotrast Exposure: Establishment of A New Cell Line (Mz–Hep–1)

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Abstract
A human hepatoma cell line, associated with thorotrast exposure, from an hepatitis B markernegative patient was established as a permanent cell line (Mz–Hep–1) in tissue culture. Histology of the primary tumor, as well as phase contrast, transmission and scanning electron microscopy of the cultured cells showed typical characteristics of liver cells. Mz–Hep–1 cells secreted complement components (C2, C3, C4), carcinoembryonic antigen, lactate dehydrogenase, chymotrypsin, haptoglobin and retinol–binding protein and expressed HLA–, transferrin–, blood group B–related determinants and complement component C5 and carcinoembryonic antigen on their cell surface. Mz–Hep–1 cells represent the first human hepatoma cell line, which is strongly associated with a carcinogen.