Isolation and characterization of nonhistone chromosomal protein C-14 which stimulates RNA synthesis
- 1 May 1977
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 16 (11) , 2384-2389
- https://doi.org/10.1021/bi00630a012
Abstract
The nonhistone chromatin protein, C-14, was extracted from chromatin of Novikoff hepatoma ascites cells and isolated in high purity as shown by its migration as a single dense spot on 2-dimensional polyacrylamide gels. Its mobility on sodium dodecyl sulfate gels is consistent with a MW of .apprx. 70,000. The amino acid composition shows that protein C-14 has an acidic:basic amino acid ratio of 1.8. Its amino terminal amino acid is lysine. Protein C-14 stimulated the incorporation of [3H]UMP into RNA by .apprx. 30% when added to naked DNA and homologous RNA polymerase I. A 30% stimulation of [3H]UMP incorporation into RNA was also found when protein C-14 was added to an Escherichia coli RNA polymerase system containing either E. coli or Novikoff hepatoma DNA.This publication has 1 reference indexed in Scilit:
- Template properties of liver chromatinJournal of Molecular Biology, 1966