Long-Term Behavioral Recovery in Parkinsonian Rats by an HSV Vector Expressing Tyrosine Hydroxylase
- 25 November 1994
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 266 (5189) , 1399-1403
- https://doi.org/10.1126/science.266.5189.1399
Abstract
One therapeutic approach to treating Parkinson's disease is to convert endogenous striatal cells into levo-3,4-dihydroxyphenylalanine (L-dopa)-producing cells. A defective herpes simplex virus type 1 vector expressing human tyrosine hydroxylase was delivered into the partially denervated striatum of 6-hydroxydopamine-lesioned rats, used as a model of Parkinson's disease. Efficient behavioral and biochemical recovery was maintained for 1 year after gene transfer. Biochemical recovery included increases in both striatal tyrosine hydroxylase enzyme activity and in extracellular dopamine concentrations. Persistence of human tyrosine hydroxylase was revealed by expression of RNA and immunoreactivity.Keywords
This publication has 57 references indexed in Scilit:
- Long-term correction of rat model of Parkinson's disease by gene therapyNature, 1993
- Microdialysis Monitoring of 3,4‐Dihydroxyphenylalanine Accumulation After Decarboxylase Inhibition: A Means to Estimate In Vivo Changes in Tyrosine Hydroxylase Activity of the Rat Locus CeruleusJournal of Neurochemistry, 1993
- Biochemical and behavioral recovery in a rodent model of Parkinson's disease following stereotactic implantation of dopamine-containing liposomesExperimental Neurology, 1992
- A latent, nonpathogenic HSV-1-derived vector stably expresses β-galactosidase in mouse neuronsNeuron, 1990
- Use of Microdialysis for Monitoring Tyrosine Hydroxylase Activity in the Brain of Conscious RatsJournal of Neurochemistry, 1990
- Isolation and characterization of the human tyrosine hydroxylase gene: identification of 5' alternative splice sites responsible for multiple mRNAsBiochemistry, 1987
- RNA Complementary to a Herpesvirus α Gene mRNA Is Prominent in Latently Infected NeuronsScience, 1987
- FETAL NEURONAL GRAFTS IN MONKEYS GIVEN METHYLPHENYLTETRAHYDROPYRIDINEThe Lancet, 1986
- The immunocytochemical detection of Herpes simplex virus in cervical smears—a valuable technique for routine useThe Journal of Pathology, 1984
- Demonstration of a pallidostriatal pathway by retrograde transport of HRP-labeled lectinBrain Research, 1981