Phenotypic Effects of Isolated pRiA4 TL-DNA rol Genes in the Presence of Intact TR-DNA in Transgenic Plants of Solanum dulcamara L.
- 1 October 1991
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 42 (10) , 1279-1286
- https://doi.org/10.1093/jxb/42.10.1279
Abstract
The effect of the rol genes, together with the TR-DNA of pRiA4 on the phenotype of Solanum dulcamara plants, was analysed. Plants transformed by Agrobacterium strain BN1010: : rol A ( rol A 7plus; TR + ) exhibited severe leaf wrinkling, whereas plants transformed by strain BN1010: :rolC (ro/C + TR + ) had a typical ‘hairy root’ phenotype. Leaf discs excised from these latter plants produced roots on hormone-free medium. BN1010: : rol ABC ( rol ABC + TR + ) transformed plants had an exaggerated transformed phenotype. Some of the BN1010: : rol ABC transformants had positively geotropic root growth which correlated with the presence of multiple copies of the TR-DNA. S. dulcamara plants, transformed by the TR-DNA region only, exhibited epinasty. Scanning electron microscopy of plants containing various regions of agropine Ri T-DNA revealed that transformation causes changes in basic plant siructure.Keywords
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