Cloning of Plasmodium falciparum protein disulfide isomerase homologue by affinity purification using the antiplasmodial inhibitor 1,4-bis{3-[N -(cyclohexyl methyl)amino]propyl}piperazine 1
- 8 November 2000
- journal article
- Published by Wiley in FEBS Letters
- Vol. 484 (3) , 246-252
- https://doi.org/10.1016/s0014-5793(00)02170-0
Abstract
A series of 10 1,4-bis(3-aminopropyl)piperazine compounds was found to display antiplasmodial activity with 50% growth inhibition between 30 and 250 nM, on three Plasmodium falciparum strains differently sensitive to chloroquine. By affinity chromatography using one of these compounds, a 52-kDa protein was isolated from P. falciparum, microsequenced and cloned. It corresponded to a single copy gene encoding a 453 amino acid protein displaying the typical features of protein disulfide isomerases, a thiol metabolizing enzyme belonging to the thiol: disulfide oxidoreductase superfamily, which was not previously described in malarial species.Keywords
This publication has 38 references indexed in Scilit:
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- Molecular Cloning and Characterization of a Putative Glutathione Reductase Gene, the PfGR2 Gene, from Plasmodium falciparumEuropean Journal of Biochemistry, 1996
- Molecular characterization of the glutathione peroxidase gene of the human malaria parasite Plasmodium falciparumMolecular and Biochemical Parasitology, 1996
- The Dynamic Organisation of the Secretory Pathway.Cell Structure and Function, 1996
- Plasmodium falciparum glutathione reductase exhibits sequence similarities with the human host enzyme in the core structure but differs at the ligand-binding sitesMolecular and Biochemical Parasitology, 1995
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994
- Protein disulphide isomerase: building bridges in protein foldingTrends in Biochemical Sciences, 1994
- Codon usage in Plasmodium falciparumMolecular and Biochemical Parasitology, 1988
- Human Malaria Parasites in Continuous CultureScience, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970