Rate of release of hepatic triacylglycerol into serum in the starved rat

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Abstract
After an i.v. injection of a pulse of [U-14C]palmitate to starved rats, the time-dependent radioactivity profiles were determined in the triacylglycerol (triglyceride) of hepatic microsomal fractions, floating fat, mitochondria and nuclei. The profile of activity in serum gave a value of 0.08 mg/min per 100 g body wt for the irreversible disposal rate of triacylglycerol from serum. This value, combined with the previously estimated rate of movement of triacylglycerol from serum to liver, and the reported rate from intestine to serum, gave a caluculated value of 0.35 mg/min per 100 g body wt for release rate of triacylglycerol from liver to serum. The rate of release of hepatic triacylglycerol into serum was also measured by the widely used Triton WR-1339 method. The rate obtained with this technique (0.15 mg of triacylglycerol/min per 100 g body wt) was identical with that reported previously. During the interval from 45 min to 3 h after ethanol administration this rate increased to 0.18 mg/min per 100 g body wt. Apparently the use of Triton underestimates the true rate of movement of triacylglycerol from liver to serum.