Somatic Antigens of Pseudomonas aeruginosa

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Abstract
An O-antigenic lipopolysaccharide, manifesting a high serological activity in the reaction of passive haemag-glutination and its inhibition, was isolated from Pseudomonas aeruginosa O:6 cells (Lanyi). Splitting-off of the lipid component by mild acid hydrolysis resulted in the polysaccharide deprived of O-specific activity. The polysaccharide chain of the lipopolysaccharide was made up exclusively of the residues of amino sugars, among which have been identified: 2-acetamido-2-deoxy-d-galactose (GalNAc), 2-acetamido-2,6-dideoxy-d- glucose (QuiNAc), 2-acetamido-2,6-dideoxy-d-galactose (FucNAc) as well as 2,3-diacetamido-2,3-dideoxy-d-glucuronic acid [Glc(NAc)2A] found naturally for the first time. The principal methods of establishing the polysaccharide structure were 13C nuclear magnetic resonance, methylation analysis and solvolysis with hydrogen fluoride. Depending on solvolysis conditions, a disaccharide or a trisaccharide containing the diacetamidouronic acid residue was formed. From the results obtained it followed that the polysaccharide chain of the O-antigenic Ps. aeruginosa O:6 lipopolysaccharide represents an acidic hexosaminoglycan constructed of the repeating tetrasaccharide units of the following structure:→4)dGalNAc(αl→4)dGlc(NAc)2A(β1 →3)dFucNAc(α1→3)dQuiNAc(α1→.