A RADIOIMMUNOASSAY FOR HUMAN PITUITARY LUTEINISING HORMONE USING PAPER CHROMATO‐ELECTROPHORESIS

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Abstract
Summary: A radioimmunoassay is described for the measurement of human pituitary luteinising hormone in small volumes of unextracted plasma. The 2nd International Reference Preparation of Human Menopausul Gonadotrophin and a preparation of purified human pituitary luteinising hormone have been used as the assay standards, and the latter has been radioactively labelled with 131I and 125I for use as the tracer antigen. Separation of bound and free labelled antigen has been achieved by the technique of paper chromato‐electrophoresis, and an internal standard has been used to reduce variability between assays.Data are presented bearing on the specificity, accuracy, precision and sensitivity of the method. Normal values in plasma were found to be as follows:(a) in 46 eugonadal males 2·25 ± 0·16 International Units (I.U.) per 100 ml. (mean ± standard error);(b) in normal females during the menstrual cycle the mean level during the follicular phase was 2·0 International Units per 100 ml, during the luteal phase 1·5 International Units per 100 ml, ami at mid‐cycle 8·1 International Units per 100 ml. (4 subjects);(c) in 14 post‐menopausal females, 7·0 ± 1·2 International Units per 100 ml.;(d) in 25 prepubertal children, < 0·3 to 1·3 International Units per 100 ml.

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