DNA sequence preferences for an intercalating porphyin compound revealed by footprinting
Open Access
- 1 January 1987
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 15 (5) , 2221-2234
- https://doi.org/10.1093/nar/15.5.2221
Abstract
The DNA sequence preferences of the compound peso -tetra- (4-N-methyl (pyrldyl) porphyrin and Its nickel complex have been Investigated by means of footprinting experiments on several DNA fragments, using DNAase I and nrlcrococcal nuclease as footprinting agents. A complex pattern of both AT and GC-protected sites was found. Ligand-Induced long-range conformational changes were Inferred 1n several Instances to be related to the observed large-scale blockages of enzymatic cutting.Keywords
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