Relationship Between Extractable Chlorophyll and an in Situ Method to Estimate Leaf Greenness
Open Access
- 1 December 1987
- journal article
- Published by American Society for Horticultural Science in HortScience
- Vol. 22 (6) , 1327
- https://doi.org/10.21273/hortsci.22.6.1327
Abstract
Traditional quantification of leaf chlorophyll requires destructive sampling of leaves and tedious extraction in 80% acetone (1,2), whereas N, N-dimethylformamide (DMF) is an alternative solvent that does not require tissue grinding (3). Minolta has recently marketed a portable meter (SPAD-501) for a rapid and nondestructive estimate of leaf greenness. Earlier results indicate a weak but significant correlation (r2 = 0.48) between SPAD readings and leaf chlorophyll extracted in acetone from data pooled from 22 species (5). The purpose of this study was to address three questions: a) is there a species-specific relationship between the SPAD readings and extractable chlorophyll; b) is the relationship of SPAD readings stronger when chlorophyll is expressed on a leaf area-or fresh-weight basis; and c) what is the consistency between two individual SPAD-501 instruments?Keywords
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