Effect of Ethrel and Ceratocystis fimbriata on the Synthesis of Fatty Acids and 6-Methoxy Mellein in Carrot Root

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Abstract
The rate of incorporation of (14)C from acetate-1-(14)C into fatty acids by carrot root discs, 18 hours after inoculation with Ceratocystis fimbriata, was 9-fold greater than that in freshly cut discs. The rate in discs treated with water or Ethrel was 3-fold greater. The rate of incorporation of (14)C from glucose-U-(13)C into fatty acids was 3-fold greater 18 hours after any of the above treatments. The rate of (14)C incorporation from malonate-2-(14)C into fatty acids 24 hours after inoculation with C. fimbriata or treatment with water was 25 and 60%, respectively, of that in freshly cut discs. Linoleic acid was the principal fatty acid in carrot root, but incorporation of (14)C from acetate-1-(14)C into the acid was low until 18 hours after inoculation with C. fimbriata or treatment with Ethrel. Turnover rates of the fatty acids appeared low and were similar for all treatments.There was little or no incorporation of (14)C from acetate or malonate into 3-methyl-6-methoxy-8-hydroxy-3,4-dihydroisocoumarin (6-methoxy mellein) in fresh carrot discs or in discs treated with water, but label was incorporated rapidly in discs inoculated with C. fimbriata or treated with Ethrel. The rate of incorporation of label from acetate into fatty acids was severalfold higher than into 6-methoxy mellein, but the rate of incorporation of label from malonate was approximately equal into fatty acids and 6-methoxy mellein 24 hours after inoculation. The rate of fatty acid synthesis does not appear directly related to the accumulation of 6-methoxy mellein.