RAPID TRANSFORMATION OF CELLS BY ROUS SARCOMA VIRUS
- 1 June 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 63 (2) , 318-325
- https://doi.org/10.1073/pnas.63.2.318
Abstract
The conditions for synchronous transformation of chick embryo cells by infection with Rous sarcoma virus are studied. Two factors, the treatment of cells with DEAE-dextran and the use of cells which grow rapidly following virus infection, are found to be most important. Under the conditions described, the Schmidt-Ruppin strain of Rous sarcoma virus at a multiplicity of higher than 5 induces morphological alteration in about 90 per cent of the cell population within 24 hours after infection. The alteration in the morphology is accompanied by acquisition of the ability of cells to grow in agar medium and by the increased rate of incorporation of thymidine and uridine into nucleic acids.Keywords
This publication has 15 references indexed in Scilit:
- Studies on the fixation and development of cellular transformation by Rous sarcoma virusVirology, 1968
- Proteins of Rous sarcoma virusVirology, 1968
- The fate of the DNA of adenovirus type 12 in baby hamster kidney cells.Proceedings of the National Academy of Sciences, 1968
- Early release of growth inhibition in cells infected with Rous sarcoma virus.Proceedings of the National Academy of Sciences, 1968
- Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals.Proceedings of the National Academy of Sciences, 1966
- Transformation by Rous Sarcoma Virus: A Requirement for DNA SynthesisScience, 1965
- Avian Tumor VirusesPublished by Elsevier ,1965
- ANALYSIS OF THE DEFECTIVENESS OF ROUS SARCOMA VIRUS, II. SPECIFICATION OF RSV ANTIGENICITY BY HELPER VIRUSProceedings of the National Academy of Sciences, 1964
- A VIRUS IN CHICK EMBRYOS WHICH INDUCES RESISTANCE IN VITRO TO INFECTION WITH ROUS SARCOMA VIRUSProceedings of the National Academy of Sciences, 1960
- An analysis of the assay of rous sarcoma cells in vitro by the infective center techniqueVirology, 1960