A New Method of Studying Temperature Dependence and the Effect of Mobile Phase Composition on the Retention Mechanism in Reversed Phase Liquid Chromatography

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Abstract
In this work, a rapid procedure to examinate the effect of temperature and eluent composition on thermodynamic properties in high performance liquid chromatography is presented. The use of an experimental design is proposed to study thermodynamic solution property trends for ten Benzodiazepines. Enthalpies and entropies of transfert (mobile to stationary phase) are calculated by evaluation of Van't Hoff plots. For all cases examined enthapies of transfert are negative. These data showed that the entropic contribution to retention becomes more significant as the solvant polarity decreases. Enthalpy-enthropy compensation behavior is tested for varying mobile phase composition.