Analysis of Modified Deoxynucleosides by Electrospray Ionization Mass SPECTROMETRY

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Abstract
Electrospray mass spectra for selected modified deoxynucleosides and deoxynucleoside monophosphates have been determined. Protonated molecular ions are abundant in the positive ion spectrum, while (M-H) appears in the negative ion spectrum. However, fragment ion intensities are usually low in both spectra. Conditions which promote collision-induced dissociation within the electrospray source facilitate fragment ion formation, and the intensity of BH2 + and S+ (positive ion spectrum) and (M-BH) and B (negative ion spectrum) are enhanced by increasing the skimmer cone voltage. MH+ was detected with as little as 3 pmol of deoxynucleoside, and the protonated molecular ion intensity is linear with respect to analyte concentration over two orders of magnitude.