PROBENECID-INDUCED EFFECTS ON BILE-FLOW AND BILIARY-EXCRETION OF OUABAIN-H-3

  • 1 January 1976
    • journal article
    • research article
    • Vol. 223  (1) , 96-106
Abstract
Probenecid-induced effects on bile flow and biliary excretion of exogenous compounds, e.g., 3H-ouabain were investigated on bile-fistula rats. Clearance measurements of 14C-mannitol under increased doses of probenecid gave evidence that the drug acts at the canalicular site only. Biliary excretion of radioactivity after 14C-probenecid administration was saturated at a dosage of 100 mg/kg. Probenecid exerts its choleretic effect by osmotic activity in a bile acid-like manner. This is confirmed by a close positive correlation between the excretion of radioactivity after treatment with 14C-probenecid and bile flow. The effects of probenecid on the biliary excretion of exogenous compounds e.g., ouabain, imply choleresis along with inhibitory effects on transport systems. As a result of both effects the biliary excretion of ouabain remains unchanged within a wide range of probenecid doses.