CD39 is incorporated into plasma microparticles where it maintains functional properties and impacts endothelial activation

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Abstract
Summary: Plasma microparticles (MPs, Entpd1 (Cd39) deletion in mice produced a pro‐inflammatory phenotype associated with quantitative and qualitative differences in the MP populations, as determined by two dimensional‐gel electrophoresis, western blot and flow cytometry. Entpd1‐null MPs were also more abundant, had significantly higher proportions of platelet‐ and endothelial‐derived elements and decreased levels of interleukin‐10, tumour necrosis factor receptor 1 and matrix metalloproteinase 2. Consequently, Cd39‐null MP augment endothelial activation, as determined by inflammatory cytokine release and upregulation of adhesion molecules in vitro. In conclusion, CD39 associates with circulating MP and may directly or indirectly confer functional properties. Our data also suggest a modulatory role for CD39 within MP in the exchange of regulatory signals between leucocytes and vascular cells.