Gas chromatographic determination of toluene in tissue by using a head space method.

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Abstract
A head space method was studied for the quantitative determination of toluene in tissues by gas chromatography (GC). The method may be summarized as follows. Tissue was minced by extruding it from a plastic syringe into an equilibration vial containing water, sodium hydroxide, olive oil and an internal standard (ethylbenzene). As a standard sample, another equilibration vial having the same components plus authentic toluene but without tissue was used. Both vials were rapidly sealed with Teflon-rubber packing, and then shaken at 35.degree. C for 2.5 h in a water bath. One milliliter of warmed head space gas was subjected to CG. The concentration of toluene in tissue was calculated by comparing T/I (T, peak height of toluene; I, peak height of internal standard) of the tissue sample with that of the standard sample. This method was useful for determining toluene in the range of 0.002-10 mg/g in tissue.

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