Chloride flux in rabbit kidney tubules in vitro

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Abstract
Renal tubules from suspensions prepared using collagenase were analyzed for concentration of Cl in the tissue and exchange rate of 36C1. Neither the concentration of Cl in tissue nor the exchange rate of 36Cl was altered when the concentration of K in the bathing medium was varied over a wide range. Although the addition of ouabain, an inhibitor of active transport, raised the concentration of Cl in tissue, no significant effect on the rate of exchange of 36C1 was observed. Similar findings with respect to tissue Cl concentration were observed in single fragments of proximal tubules obtained by micro-dissection. These data are interpreted as indicating that Cl is not dis -tributed in the proximal nephron in accordance with the Donnan relationship, nor is it affected by manipulations which are assumed to alter transmembrane potential difference. Cl in renal tissue was kinetically heterogeneous and exchanged at least 2 different rates. The transcellular flux of Cl was considerably less than that of Na (previously reported) under similar conditions. This findings is inconsistent with the view that the flux of Na represents transtubular transport of NaCl.

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