Evidence for Oxidative Stress during in vitro Dialysis

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Abstract
The evidence for oxidative stress during haemodialysis is controversial. We therefore examined markers of oxidative stress and lipid peroxidation using an in vitro dialysis circuit. A unit of fresh blood (500 ml) therapeutically removed from each of 7 haemochromatosis patients was oxygenated and circulated for 4 h at 37 °C through a cuprophane dialyser (Clirans C08) against saline dialysate (1,000 ml recirculating; +FIL group). In a second series of experiments (n = 7), the dialyser was omitted from the circuit (-FIL group). Concentrations of anti-oxidants and malondialdehyde (MDA) were measured at 7 time points during the study. Blood thiol concentrations decreased by 25.6% in the +FIL group (p 0.05; group comparison, p = 0.006). There were no significant differences between the groups, for the lipid-soluble anti-oxidants α-tocopherol, retinol and β-carotene. Plasma MDA concentrations increased in both circuits (p

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