Affinity alkylation labels two subunits of the reduced acetylcholine receptor from mammalian muscle.
- 1 October 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (10) , 4685-4688
- https://doi.org/10.1073/pnas.74.10.4685
Abstract
The acetylcholine receptor from denervated rat skeletal muscle was purified by affinity chromatography and, after reduction, was treated with the affinity alkylating agent 4-(N-maleimido)benzyltri[3H]methylammonium iodide. The receptor specifically incorporated approximately 1 mol of alkylating agent/mol of 125I-labeled .alpha.-bungarotoxin bound. Analysis of the labeled receptor by polyacrylamide gel electrophoresis in sodium dodecyl sulfate showed that 2 subunits were labeled; their apparent MW were 45,000 and 49,000. The affinity reagent labeled a 2nd site for acetylcholine binding in the muscle receptor that was not labeled in receptors from Electrophorus or Torpedo.This publication has 19 references indexed in Scilit:
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