Association of a cGMP-binding activity with nuclei isolated from amoebae of Dictyostelium discoideum and Polysphondylium violaceum

1 February 1992

journal article
Published by Canadian Science Publishing in Biochemistry and Cell Biology

Vol. 70 (2) , 169-173
https://doi.org/10.1139/o92-025

Abstract

When vegetative and early slug stage amoebae of Dictyostelium discoideum or Polysphondylium violaceum were lysed by filter breakage in a nuclear isolation buffer not containing detergents, substantial levels of a cGMP-binding activity with slow-dissociation kinetics were detected. After fractionation by centrifugation, 50% or more of this binding activity was associated with isolated nuclei. In addition, with Polysphondylium cells, the fraction of stable, nuclear-associated binding activity appeared to increase during cell aggregation. These results support the idea that cGMP might function in the nucleus during early development.Key words: cGMP-binding activity, nuclear isolation, Dictyostelium discoideum, Polysphondylium violaceum.

Keywords

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