Myeloma Globulins of Plasma-Cell Neoplasms in Inbred Mice. I. Immunoelectrophoresis of Serum, With Rabbit Antibodies Prepared Against Microsome Fractions of the Neoplasms

Publisher Website
Google Scholar
Abstract
With the technique of agar-gel immunoelectrophoresis, a study was made of serum from normal BALB/c mice and from BALB/c mice bearing different plasma-cell neoplasms. The precipitating antibody used was developed in rabbits after immunization with washed microsomes obtained from transplants of the plasma-cell neoplasms. Three different neoplasms were employed separately: MPC-1, MPC-2, and Adj-PC-5. Each of these antibodies formed different patterns of precipitation with electrophoresed normal serum. Two arcs were developed: One, designated System I (SI), appeared to be identical with γ2-globulin, and the second, designated System II (SII), was a β-globulin, the probable murine counterpart for the β2A-globulin of man. The myeloma globulins appeared as anomalies of the SI and SII arcs in the form of crescent-shaped precipitates. The myeloma globulins of 13 different plasma-cell neoplasms of strain BALB/c were studied; 2 belonged to SI, 9 to SII, 1 was antigenically related to both, and 1 produced an SI and SII myeloma globulin. A remarkable degree of physical heterogeneity of the myeloma globulins was observed among the various cases within the systems. Further, many of the anomalous arcs which characterized the myeloma globulins of individual neoplasms were asymmetrical and were interpreted to indicate that the myeloma globulins made by individual neoplasms were also heterogeneous. Two stable protein-producing transplant lines were established from a single primary host, Adj-PC-6.