Transgenic Production of a Variant of Human Tissue-Type Plasminogen Activator in Goat Milk: Generation of Transgenic Goats and Analysis of Expression
- 1 September 1991
- journal article
- research article
- Published by Springer Nature in Nature Biotechnology
- Vol. 9 (9) , 835-838
- https://doi.org/10.1038/nbt0991-835
Abstract
We report the first successful production of transgenic goats that express a heterologous protein in their milk. The production of a glycosylation variant of human tPA (LAtPA - longer acting tissue plasminogen activator) from an expression vector containing the murine whey acid promoter (WAP) operatively linked to the cDNA of a modified version of human tPA was examined in transgenic dairy goats. Two transgenic goats were identified from 29 animals born. The first animal, a female, was mated and allowed to carry the pregnancy to term. Milk was obtained upon parturition and was shown to contain enzymatically active LAtPA at a concentration of 3 μg/ml.Keywords
This publication has 10 references indexed in Scilit:
- High-level synthesis of a heterologous milk protein in the mammary glands of transgenic swine.Proceedings of the National Academy of Sciences, 1991
- Composition of milk of native Greek goats in the region of metsovoSmall Ruminant Research, 1991
- The mammary gland as a bioreactor: Production of foreign proteins in milkProtein Expression and Purification, 1990
- High-level expression of biologically active human alpha 1-antitrypsin in the milk of transgenic mice.Proceedings of the National Academy of Sciences, 1990
- Porcine growth hormone gene expression from viral promoters in transgenic swineAnimal Biotechnology, 1990
- Collection and transfer of microinjectable embryos from dairy goatsTheriogenology, 1990
- A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice.Proceedings of the National Academy of Sciences, 1988
- Production of transgenic rabbits, sheep and pigs by microinjectionNature, 1985
- Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.Proceedings of the National Academy of Sciences, 1980
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976