RADIOIMMUNOASSAY FOR PHENCYCLIDINE
- 1 January 1979
- journal article
- research article
- Vol. 25 (3) , 547-557
Abstract
A sensitive and specific radioimmunoassay was developed for the drug phencyclidine (PCP). Levels as low as 50 pg (200 femtomol) of PCP can be detected. The specificity of the antisera [formed in rabbits] was investigated by inhibiting the [3H]PCP-anti PCP reaction with the parent drug, metabolites, and other related compounds. Three urinary metabolites, 4-phenyl-4-piperidinocyclohexanol, 1-(1-phenylcyclohexyl)-4-hydroxypiperidine and 1-phenylcyclohexylamine inhibit 2, 5 and 0.002% as effectively as PCP, respectively. Plasma [human] samples supplemented with PCP (1.2-10,000 ng/ml) can be analyzed directly by this procedure. While the legal use of PCP [1-(1-phenylcyclohexyl)piperidine, Angel Dust] is as an analgesic and anesthetic in veterinary medicine, it has also found increasing popularity among drug users.This publication has 4 references indexed in Scilit:
- Effects of urine acidification on plasma and urine phencyclidine levels in overdosageClinical Pharmacology & Therapeutics, 1977
- Detection and quantitation of phencyclidine in blood by use of [(2)H(5)] phencyclidine and select ion monitoring applied to non-fatal cases of phencyclidine intoxication.Clinical Chemistry, 1976
- The Synthesis of Phencyclidine and Other 1-ArylcyclohexylaminesJournal of Medicinal Chemistry, 1965
- IMMUNOASSAY OF ENDOGENOUS PLASMA INSULIN IN MANJournal of Clinical Investigation, 1960