CHICKEN FETAL AND ADULT ANTIGEN EXPRESSION ON ERYTHROLEUKEMIA-CELLS BEFORE AND AFTER INDUCED-DIFFERENTIATION

  • 1 January 1982
    • journal article
    • research article
    • Vol. 42  (11) , 4625-4630
Abstract
Avian erythroblastosis virus strain R (AEV)-transformed, cloned erythroleukemia cells from 3 different ages of SC strain chickens were analyzed before and after differentiation induced by 1.0 mM butyric acid for expression or chicken fetal antigens (CFA) and chicken adult antigens (CAA) and for Hb expression. Immunofluorescent analyses show the loss of individual CFA determinants from erythroleukemia cells with induced differentiation, although there appeared to be no correlation between CFA loss and onset of Hb production. Erythroleukemia cells were examined by cell surface labeling followed by immunoprecipitation with antisera specific to CFA and CAA. Erythroleukemia cells expressed CFA and CAA on their membranes that are not reported to be expressed by the target cell of AEV. The expression of CAA and the enhanced expression of CFA by erythroleukemia cells may be due to limited cellular differentiation, alterations in regulatory controls of genes coding for CFA and CAA, or increased levels of production of previously undetected CFA and CAA following AEV transformation. Control and induced erythroleukemia cells expressed CFA and CAA that differed both quantitatively and qualitatively from normal erythroid cells. Molecular weight variations of CFA and CAA observed in the erythroleukemia cells may represent glycolyzation differences between AEV-transformed cells and normal erythroid cells.