THE EFFECT OF HYDROXYUREA ON MEIOSIS AND GENETIC RECOMBINATION IN THE FUNGUSCOPRINUS LAGOPUS

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Abstract
Treatment with hydroxyurea (HU) inhibits fruit body development in C. lagopus (sensu Lewis). The 2 most sensitive meiotic stages are mid-late premeiotic S phase and the pachytene-diplotene period. A 2 or 4 h treatment at mid-late premeiotic S phase arrests fruit body development. If the same treatment is given at pachytene and diplotene, the fruit body completes meiosis but the spores produced are inviable. The spores from fruit bodies treated at pachytene apparently are normal whereas those treated at diplotene are empty because the 4 nuclei remain in the basidium. A 2 or 4 h treatment with HU during karyogamy causes a decrease in spore viability and a dramatic increase in recombination frequency. The same treatment at metaphase II or later stages causes little damage to fruit body development or spore viability. The effects of HU on the meiotic cell cycle suggest that the drug exerts its effect by inhibiting the synthesis of deoxynucleotides.

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