DIFFERENTIAL REGULATION OF ALPHA-LACTALBUMIN AND CASEIN MESSENGER-RNAS IN MAMMARY TISSUE

  • 1 January 1978
    • journal article
    • research article
    • Vol. 38  (9) , 2694-2699
Abstract
Rat caseins were characterized with respect to MW and carbohydrate and amino acid content. Messenger RNA was extracted fron rat mammary glands at 5, 10, 15 and 20 days of pregnancy and at 2, 8 and 15 days of lactation and translated in a wheat germ cell-free system. Nascent [3H]casein and .alpha.-[3H]lactalbumin were precipitated separately with specific antibodies and identified by their mobilities on sodium dodecyl sulfate:acrylamide gels and by competition with unlabeled casein and .alpha.-lactalbumin, respectively. Casein messenger rose continuously from day 5 of pregnancy day 15 of lactation. In contrast, .alpha.-lactalbumin mRNA levels remained low during pregnancy but rose markedly during lactation.

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