Effect of Gamma Radiation on DDT Resistance and DDT-Dehydrochlorinase Levels in Musca domestica12

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Abstract
During DDT-resistant house fly development, the amount of DDT-dehydrochlorinase increased sharply during the larval stages, decreased markedly at pupation, and increased again upon adult eclosion. None was detected in eggs or in day-old larvae. The protein synthesis inhibitors puromycin, actinomycin D, and chloramphenicol reduced the levels of α-glycerophosphate dehydrogenase and DDT-dehydrochlorinase in teneral adult flies. In 4–5-day-old larvae exposed when less than 24 h old to 6, 12, 18, or 24 krad of gamma radiation, DDT-dehydrochlorinase specific activity decreased as the radiation dose increased. After 1 additional day of development, DDT-dehydrochlorinase activity decreased in both irradiated and nonirradiated larvae but the decrease was less in irradiated larvae exposed to greater doses of radiation. DDT-dehydrochlorinase specific activity in adults irradiated as 5 to 6-day-old pupae with the aforementioned doses of gamma radiation was unaffected by radiation. However, gamma-irradiated adults showed slight increased resistance to topically applied DDT.

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