Biosynthesis of bacterial glycogen: genetic and allosteric regulation of glycogen biosynthesis in Salmonella typhimurium LT-2

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Abstract
Structural gene mutants of the glycogen biosynthetic enzymes ADP glucose pyrophosphorylase (glgC) and glycogen synthase (glgA) were isolated and partially characterized. The cotransduction frequencies of these genes with the aspartic semialdehyde dehydrogenase (asd) and glycerol-3-phosphate dehydrogenase (glpD) genes suggested the unambiguous gene order of glpD glgA glgC asd. The results of the 3-factor cross glpD- glgA- glgC+ .times. glpD+ glgA+ glgC- were consistent with the proposed order. A simultaneous and approximately equivalent derepression of the glgC, glgA and glgB (branching enzyme) gene products was observed in the late log-early stationary phase of growth on enriched media. These results are consistent with the coordinately regulated synthesis of the 3 glycogen biosynthetic enzymes in S. typhimurium.