Assessment of transport and isolation methods for gonococci.

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Abstract
Urethral discharge from men was diluted to give heavy and light inocula and cultured on 7 different solid culture media, including 2 transport/isolation media, or held in 3 types of semi-solid transport medium for varying periods and then cultured. The amount of growth was quantitated and the performance of the different systems compared. Fresh nonselective media were best, with up to 2 failures in 254 cultures on each medium. With selective media there were 9-23 failures with heavy inocula and 22-47 failures with dilute inocula. For Transgrow or the Jembec system, incubation before holding at ambient temperature was better than holding followed by incubation. Transport media yielded good results if cultures were set up within 6 h; only minor losses occurred after 24 h.