Effects of Phosphate Limitation of Growth on the Cell-wall and Lipid Composition of Saccharomyces cerevisiae

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Abstract
Summary: The T incompatibility group plasmid R394 can mobilize the chromosome of Proteus mirabilis strain pm5006. It transferred relatively large segments, corresponding to at least 20 min on the D plasmid chromosomal map of the organism. The frequency of recombination for a large number of selected markers was nearly constant at 5 × 10−6 per donor cell and it is concluded that mobilization takes place from a number of chromosomal sites. All recombinants were R+ and displayed all properties of the plasmid. By analysing crosses for co-inheritance frequencies of unselected markers, a number of chromosomal loci were assembled in linear array. Linkage between markers at the ends of this linkage group was established to markers at the respective termini of the existing D plasmid linkage group. This established a composite circular linkage map of genes of the P. mirabilis strain pm5006 chromosome.