A cleavable cross‐linking reaction for protein carboxyl groups

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Abstract
L-(+)-tartaric acid dihydrazide was coupled to the purple membrane from Halobacterium halobium with a water soluble carbodiimide. Gel electrophoresis analysis of the product revealed the formation of bacteriorhodopsin dimers, trimers and higher polymers. Most of the cross-links were removed by treatment with papain, demonstrating involvement of the carboxyl-rich carboxyl-terminal region of bacteriorhodopsin in the reaction. The cross-links were cleavable by periodate oxidation.