Protective Effect of Broccoli, Onion, Carrot, and Licorice Extracts against Cytotoxicity of N-Nitrosamines Evaluated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide Assay
- 6 January 1998
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 46 (2) , 585-589
- https://doi.org/10.1021/jf970636i
Abstract
The protective effect of nine fruit and vegetable aqueous (H(2)O) and ethanolic (EtOH) extracts against the cytotoxicity of N-nitrosodimethylamine (NDMA), N-nitrosopyrrolidine (NPYR), N-nitrosodibutylamine (NDBA), and N-nitrosopiperidine (NPIP) was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The fruit extracts under investigation did not show a protective effect against any N-nitrosamines tested. Four vegetable extracts exhibited a protective effect (to 100% of survival) and a stimulation of cellular proliferation (>100% of survival) in decreasing order against NDMA and NPYR: broccoli(EtOH) > onion(H2O) > carrot(EtOH) > onion(EtOH) > licorice(H2O). Decreasing orders against NDBA and NPIP were, respectively, broccoli(EtOH) > licorice(H2O) > carrot(EtOH) > onion(EtOH) and broccoli(EtOH) > carrot(EtOH) > licorice(H2O) > onion(EtOH). Thus, broccoli(EtOH) extract (19-20 mg/mL) showed greater protective effect and stimulation of cellular proliferation (160% of survival) against all N-nitrosamines studied than the other vegetable extracts tested.Keywords
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