Production of the Subdomains of the Plasmodium falciparum Apical Membrane Antigen 1 Ectodomain and Analysis of the Immune Response
Open Access
- 1 August 2004
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (8) , 4464-4470
- https://doi.org/10.1128/iai.72.8.4464-4470.2004
Abstract
The apical membrane antigen 1 of Plasmodium falciparum is one of the leading candidate antigens being developed as a vaccine to prevent malaria. This merozoite transmembrane protein has an ectodomain that can be divided into three subdomains (D I, D II, and D III). We have previously expressed a major portion of this ectodomain and have shown that it can induce antibodies that prevent merozoite invasion into red blood cells in an in vitro growth and invasion assay. To analyze the antibody responses directed against the individual subdomains, we constructed six different genes that express each of the domains separately (D I, D II, or D III) or in combination with another domain (D I+II, D II+III, or D I+III). These proteins were purified and used to immunize rabbits to raise construct-specific antibodies. We demonstrated that D I+II induced a significant amount of the growth-inhibitory antibodies active in the growth and invasion assay.Keywords
This publication has 41 references indexed in Scilit:
- Induction of Parasite Growth-Inhibitory Antibodies by a Virosomal Formulation of a Peptidomimetic of Loop I from Domain III of Plasmodium falciparum Apical Membrane Antigen 1Infection and Immunity, 2003
- Purification, Characterization, and Immunogenicity of the Refolded Ectodomain of the Plasmodium falciparum Apical Membrane Antigen 1 Expressed in Escherichia coliInfection and Immunity, 2002
- Proteolytic Processing and Primary Structure ofPlasmodium falciparum Apical Membrane Antigen-1Journal of Biological Chemistry, 2001
- Specificity of the Protective Antibody Response to Apical Membrane Antigen 1Infection and Immunity, 2001
- Immunization with Parasite-Derived Apical Membrane Antigen 1 or Passive Immunization with a Specific Monoclonal Antibody Protects BALB/c Mice against Lethal Plasmodium yoelii yoelii YM Blood-Stage InfectionInfection and Immunity, 2000
- PATHWAYS AND STRATEGIES FOR DEVELOPING A MALARIA BLOOD-STAGE VACCINEAnnual Review of Immunology, 1998
- Diversity of the vaccine candidate AMA-1 of Plasmodium falciparumMolecular and Biochemical Parasitology, 1996
- Differential localization of full-length and processed forms of PF83/AMA-1 an apical membrane antigen of Plasmodium falciparum merozoitesMolecular and Biochemical Parasitology, 1994
- Inhibitory monoclonal antibodies recognise epitopes adjacent to a proteolytic cleavage site on the RAP-1 protein of Plasmodium falciparumMolecular and Biochemical Parasitology, 1992
- Vaccination trials in rhesus monkeys with a minor, invariant, Plasmodium knowlesi 66 kD merozoite antigenParasite Immunology, 1988