Relating Structure to Function in the Beta-Propeller Domain of Dipeptidyl Peptidase IV
- 31 December 1999
- book chapter
- Published by Springer Nature
- Vol. 477, 89-95
- https://doi.org/10.1007/0-306-46826-3_8
Abstract
Point mutations in human CD26/DP IV were analysed for adenosine deaminase (ADA) binding, monoclonal antibody (mAb) binding and DP IV enzyme activity. Point mutations at either Leu294 or Val341 ablated ADA binding. Binding by mAbs that inhibit ADA binding was found to involve both Leu340 to Arg343 and Thr440/Lys441. Glu205 and Glu206 were found to be essential for enzyme activity. All residues of interest were mapped onto a model of the β-propeller domain of DP IV. These data led us to suggest that in DP IV and related peptidases ligand and antibody binding sites are non-linear and that enzyme activity depends on charged sidechains that surround the entrance to the central tunnel of the β-propeller.Keywords
This publication has 15 references indexed in Scilit:
- Two highly conserved glutamic acid residues in the predicted β propeller domain of dipeptidyl peptidase IV are required for its enzyme activityPublished by Wiley ,1999
- Fibroblast Activation Protein: A Cell Surface Dipeptidyl Peptidase and Gelatinase Expressed by Stellate Cells At the Tissue Remodelling Interface in Human CirrhosisHepatology, 1999
- The Adenosine Deaminase-Binding Region Is Distinct from Major Anti-CD26 mAb Epitopes on the Human Dipeptidyl Peptidase IV(CD26) MoleculeCellular Immunology, 1999
- Prolyl OligopeptidaseCell, 1998
- Correlation of the epitopes defined by anti-CD26 mAbs and CD26 functionMolecular Immunology, 1998
- Alterations in Structure and Cellular Localization of Molecular Forms of DP IV/CD26 during T Cell ActivationCellular Immunology, 1996
- Binding of adenosine deaminase to the lymphocyte surface via CD26European Journal of Immunology, 1994
- A new approach to protein fold recognitionNature, 1992
- MOLSCRIPT: a program to produce both detailed and schematic plots of protein structuresJournal of Applied Crystallography, 1991
- Expression of the rat CD26 Antigen (dipeptidyl peptidase IV) on subpopulations of rat lymphocytesCellular Immunology, 1991