IMMUNOCHEMICAL EVIDENCE FOR 2 3-METHYLCHOLANTHRENE-INDUCIBLE FORMS OF CYTOCHROME-P-448 IN RAT-LIVER MICROSOMES USING A DOUBLE-ANTIBODY RADIOIMMUNOASSAY PROCEDURE

  • 1 January 1983
    • journal article
    • research article
    • Vol. 23  (1) , 252-257
Abstract
Double-antibody radioimmunoassay (RIA) procedures were developed for the determination of 2 forms of cytochrome P-448 in crude liver microsomes at the picogram level. The antisera used in the RIA were produced against 2 forms of cytochrome P-448 isolated from livers of rats treated with 3,4,5,3'',4'',5''-hexachlorobiphenyl (3,4,5-HCB) and 3-methylcholanthrene (3-MC), referred to as cytochromes P-448HCB and P-448MC, respectively. The results obtained with RIA procedures were consistent with results of radial immunodiffusion analysis, sodium dodecyl sulfate/polyacrylamide gel electrophoresis and estimation of aryl hydrocarbon hydroxylase (AHH) activity. Utilizing the RIA, both forms of cytochrome P-448 were found in significant quantities in liver microsomes of rats treated with 3-MC, 3,4,5-HCB, isosafrole, and Aroclor 1254 but only in minute concentrations in untreated and phenobarbital-treated rats.

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