PG-M/versican binds to P-selectin glycoprotein ligand-1 and mediates leukocyte aggregation
- 15 November 2004
- journal article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 117 (24) , 5887-5895
- https://doi.org/10.1242/jcs.01516
Abstract
P-selectin glycoprotein ligand-1 (PSGL-1), a glycoprotein expressed on the cell surface of leukocytes, binds to selectins and mediates leukocyte rolling on the vascular endothelium. Here we report that PSGL-1 binds to the C-terminal (G3 domain) of the extracellular proteoglycan PG-M/versican. Cells transfected with PSGL-1 or a shorter form containing the binding site, or cells expressing endogenous PSGL-1 aggregate in the presence of versican or G3 product. The aggregation appears to be induced by G3 multimers that bind to PSGL-1 and form a network. Endogenous versican and/or G3-containing fragments also bind to PSGL-1 in human plasma. Removal of the endogenous G3-containing fragments reduces the effect of plasma on leukocyte aggregation. Finally, the roles of G3-containing fragments in leukocyte aggregation were confirmed in a mouse model. Taken together, our results strongly support a physiologically relevant role for PSGL-1/versican binding and may have implications in the immunoresponse.Keywords
This publication has 34 references indexed in Scilit:
- Overexpression of the C-terminal PG-M/versican domain impairs growth of tumor cells by intervening in the interaction between epidermal growth factor receptor and β1-integrinJournal of Cell Science, 2004
- Versican/PG‐M G3 domain promotes tumor growth and angiogenesisThe FASEB Journal, 2004
- G3 Domains of Aggrecan and PG-M/Versican Form Intermolecular Disulfide Bonds that Stabilize Cell−Matrix InteractionBiochemistry, 2003
- Cleavage of the Carboxyl Tail from the G3 Domain of Aggrecan but Not Versican and Identification of the Amino Acids Involved in the DegradationJournal of Biological Chemistry, 2002
- β1-Integrin-mediated Glioma Cell Adhesion and Free Radical-induced Apoptosis Are Regulated by Binding to a C-terminal Domain of PG-M/VersicanPublished by Elsevier ,2002
- The G3 Domain of Versican Inhibits Mesenchymal Chondrogenesis via the Epidermal Growth Factor-like MotifsPublished by Elsevier ,1998
- DEPOSITION OF PG-M/VERSICAN IS A MAJOR CAUSE OF HUMAN CORONARY RESTENOSIS AFTER PERCUTANEOUS TRANSLUMINAL CORONARY ANGIOPLASTYThe Journal of Pathology, 1996
- P-selectin Glycoprotein Ligand-1 Is the Major Counter-receptor for P-selectin on Stimulated T Cells and Is Widely Distributed in Non-functional Form on Many Lymphocytic CellsJournal of Biological Chemistry, 1995
- P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin.The Journal of cell biology, 1995
- Antibodies against transforming growth factor-beta 1 suppress intimal hyperplasia in a rat model.Journal of Clinical Investigation, 1994