Human cord blood long-term engrafting cells are CD34+ CD38−
- 1 May 2003
- journal article
- Published by Springer Nature in Leukemia
- Vol. 17 (5) , 960-964
- https://doi.org/10.1038/sj.leu.2402878
Abstract
There have been controversies about CD34 and CD38 expression by human cord blood (CB) stem cells. Using the newborn NOD/SCID/2-microglobulin-null mouse assay that we recently developed, we examined the in vivo engrafting capability of human CB cells. Almost all of the 4–5 months engrafting cells were found in CD34+ population. The capability of secondary reconstitution was found only in the CD34+ cells. When the CD34+ CB cells were separated into CD38- and CD38+ subpopulations and tested for engraftment, the majority of the engrafting cells were detected in the CD38- subpopulation. These findings are consistent with the results from studies of murine stem cells and strongly indicate that the phenotype of human CB stem cells is CD34+ CD38-.Keywords
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