Demonstration of anti‐nuclear antibodies in fowl sera by immunofluorescence

Abstract
The occurrence of anti-nuclear antibodies in fowl sera is described. The anti-nuclear activity was demonstrated by immunofluorescence in a small percentage (3.9%) of the sera tested. The nuclei of cultured fowl and turkey cells were stained by anti-nuclear serum, whereas the nuclei of several mammalian tissues were not. Staining of chicken kidney cell nuclei by anti-nuclear serum conjugated with fluorescein isothiocyanate was blocked by unconjugated anti-nuclear serum but not by serum without anti-nuclear activity, indicating the specificity of the reaction. Confusion between anti-nuclear staining and specific viral [fowl adenovirus] staining is discussed, with the possible association of anti-nuclear factors with disease in birds.

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