HETEROGENEITY OF HOLOCARBOXYLASE SYNTHETASE IN PATIENTS WITH BIOTIN-RESPONSIVE MULTIPLE CARBOXYLASE DEFICIENCY

  • 1 January 1985
    • journal article
    • research article
    • Vol. 37  (2) , 326-337
Abstract
Holocarboxylase synthetase activity was determined in fibroblasts of 7 patients with the neonatal form of biotin-responsive multiple carboxylase deficiency. The normal Km for biotin was 15 .+-. 3 nmol/l, while in the patients the values ranged from 48-1062 nmol/l. The mean maximum velocity was 27% of normal. Differences among the values obtained for the Km for biotin and the heat stability of holocarboxylase synthetase suggested that the patients studied represented at least 4 distinct variants at the holocarboxylase synthetase locus.