A Microbiological Method for the Assay of Subtilin

Abstract
A filter paper disc method for the microbiological assay of subtilin, using Bacillus cereus 247, is descr. The method is applicable to the assay of subtilin in certain body fluids. With the proposed procedure the error of the assay is approx. [plus or minus]5-10%, depending on the no. of dilutions of the unknown falling within the range of the standard curve. The effect of such variables as pH and NaCl conc. of the medium has been detd.
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