Reciprocal Changes, Caused by Insulin and Glucagon, of Adipose Tissue Lipoprotein Lipase in Rats In Vitro

Abstract
Effects of insulin and glucagon on lipoprotein lipase activity in rat adipose tissue were studied in vitro. Adipose tissue from normal or streptozotocin-diabetic rats was incubated in Krebs-Ringer bicarbonate buffer for 60 min at 37.degree. C in the presence of heparin and lipoprotein lipase released from the tissue into the incubation medium was measured. Diabetic rats showed markedly low activities of adipose tissue lipoprotein lipase. Insulin (500 .mu.U/ml) increased lipoprotein lipase activity in adipose tissue from diabetic rats. Glucagon (500 pg/ml) decreased the enzyme activity in normal rat adipose tissue. Insulin and glucagon cause reciprocal changes of lipoprotein lipase activity in rat adipose tissue.

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