Galanin inhibits insulin secretion by direct interference with exocytosis
- 24 April 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 247 (2) , 401-404
- https://doi.org/10.1016/0014-5793(89)81379-1
Abstract
Electrically permeabilized RINm5F cells were used to study whether galanin inhibits insulin secretion distally to the generation of soluble second messengers. Ca2+-induced insulin secretion was inhibited by the neuropeptide in a dose-dependent manner. Galanin appears to act via a G-protein as persussis toxin treatment abolished the effect. GTP (100 μM), GDP (100 μM) and a low dose of GTPγS (10 μM) did not affect galanin-mediated inhibition of secretion. In contrast, at 100 μM, GTPγS attenuated and GDPβS abolished the effect of the peptide. We conclude that galanin inhibits exocytosis directly by a mechanism involving a G-protein.Keywords
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